Mirosław Nowakowski, Ph. D.
PL 85-090 Bydgoszcz, Al. Powstańców Wielkopolskich 10
phone: (+48 52) 581 69 59, fax: (+48 52) 322 44 54
e-mail: sekretariat@ihar.bydgoszcz.pl

Research Division of Root Crops in Bydgoszcz.

Department of Genetics and Breeding of Root Crops
Maria Gośka, D.Sc.
phone: (+48 52) 581 69 37
e-mail: m.goska@ihar.bydgoszcz.pl

Laboratory of Biotechnology
vacat
phone: (+48 52) 581 69 29

Objectives:

• culture and regeneration of protoplasts of sugar beet (Beta vulgaris L.) and tobacco (Nicotiana tabacum L.),
  
  

  Protoplasts of sugar beet divide and regenerate into callus and roots (left panel), whereas tobacco protoplasts develop into shoots (right panel).

  
  
• differentiation of flower organs, micro- and macrosporogenesis,
• study mechanisms involved in cell differentiation, determination of temporal and spatial expression of cell cycle genes,
• immunodetection in situ of cell antigens - proteins, proteoglicans and polysaccharides - involved in cell growth and development in vitro and in planta.
  
  

  Immunodetection of pectins in sugar beet anthers with antibodies coupled with FITC.

• in vitro micropropagation of energetic and medecinal plant species.

Laboratory of Cytogenetics and Breeding Methods
Maria Gośka, D.Sc.
phone: (+48 52) 581 69 37
e-mail: m.goska@ihar.bydgoszcz.pl

Objectives:

• induction of somatic embryogenesis and plant regeneration in vitro culture of sugar beet,
• production of dihaploid and doubled haploid lines from multigerm sugar beet populations,
  
  

  Dihaploid sugar beet plants on the regeneration medium.	Rooted sugar beet plant before transplantation into the pot.

  
  
• identification of self-compatible diploid genotypes in multigerm populations of sugar beet as the pollinators for hybrids production,
• selection of high seed quality genotypes within sugar beet O type lines,
• development of initial partial resistance of sugar beet breeding materials to Cercospora beticola Sacc.,
• in vitro micropropagation of Miscanthus x giganteus (Greef & Deu.).
• genus Beta collection (wild and cultivated beets).
  
  

  Genus Beta collection: male sterile plants of Beta maritima.

Objectives:

• research on the influence of micronutrients at different K-fertilisation on the yield of sugar beet in the cultivation without of farmyard manure,
  

Seeder Unicorn-3 with the applicator for the localized fertilization used in research.	Fertlilizing experiment on sugar beet cultivar "Polko".

• sugar beet reaction to seed and foliar application of microelements,
• usability assessment of white mustard, oil radish, tansy phacelia, flax and Solanum sisymbriifolium as a fertilising and anti-nematode factor in the cultivation of sugar beet and potato,
• assessment of drought stress tolerance of sugar beet,
• identification of the sugar beet tolerance sources of: Aphanomyces cochlioides, Rhizoctonia solani, Polymyxa betae, Cercospora beticola, Erysiphe betae,

Symptoms of Cercospora leaf spot disease resulting in great yield losses.

• the occurrence of rhizomania of sugar beet, resistance sources and elaboration of effective selection methods,
• yielding of sugar beet and density of the beet cyst-nematode (Heterodera schachtii Schmidt) population in soil after cultivation of tolerant beet varietes.

Laboratory for Quarantine Diseases and Pests of Potato
Teresa Pastuszewska, Ph.D.
phone/fax (+48 52) 581 69 32
e-mail: t.pastuszewska@ihar.bydgoszcz.pl

Objectives:

• assessment of potato breeding lines and cultivars for presence of the latent form of bacterial ring rot of potato (Clavibacter michiganensis subsp. sepedonicus),
  

Potato tuber attacked by Clavibacter michiganensis subsp. sepedonicus with characteristic „ring rot”

• characterization of bacteria Clavibacter michiganensis subsp. sepedonicus population from seed potato cultivations in country in respect to:
  – susceptibility of potato cultivars to bacterial ring rot of potato (Clavibacter michiganensis subsp. sepedonicus),
  – development of bacterial ring rot infection in potato reproduction,
• Clavibacter michiganensis subsp. sepedonicus survival period causes bacterial ring rot, on different surfaces in diverse temperature,
• study of bactericidal activity of chemical disinfectants to Clavibacter michiganensis subsp. sepedonicus causes bacterial ring rot.

Cysts of Globodera rostochiensis on potatoes roots	Warts of Synchytrium endobioticum on potatoes plant.