Professor Ewa Zimnoch-Guzowska, D.Sc.
PL 05-831 Młochów, Platanowa 19 str.
phone: (+48 22) 729 92 48, 729 90 27, 729 90 87, 729 90 88
fax: (+48 22) 729 92 47
e-mail: mlochow@ihar.edu.pl

Młochów Research Centre.

Department of Potato Genetics and Parental Lines
Professor Ewa Zimnoch-Guzowska, D. Sc.
phone: (+48 22) 729 90 89 or 729 92 48 ext. 206
e-mail: e.zimnoch-guzowska@ihar.edu.pl

Laboratory of Genetics
Henryka Jakuczun, Ph.D.
phone: (+48 22) 729 92 48 ext. 214
e-mail: h.jakuczun@ihar.edu.pl

Objectives:

• diploid breeding program of parental lines for table, processing and resistance traits,
• genetic analysis and search of new sources of quality and pathogen resistance in diploid potato,

Solanum wild species - new source of quality and resistance traits.

• cytological characterization of selected diploid clones,

Diploid potatoes on the field.

• field collection of diploid potato germplasm,
• study on inheritance of the resistance to Potato leafroll virus (PLRV).

Laboratory of Parental Lines and Breeding Methods
Bogdan Flis, Ph.D.
phone: (+48 22) 729 92 48 ext. 216
e-mail: b.flis@ihar.edu.pl

Objectives:

• development of tetraploid potato parental lines outstanding in quality and resistant to pathogens,

Parental lines suitable for chips production after cold storage.

• studies on inheritance of transgenic traits,

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Variability of morphological traits of transgenic potato lines.

• interaction studies between virus resistant transgenic lines and virus population,

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Evaluation of resistance to late blight in a detached leaflet assay.

• agronomic evaluation of transgenic potatoes,
• studies on new tetraploid sources of resistance to late blight and resistance stability,
• evaluation of combining abilities of potential tetra- and diploid potato parents for breeding purposes,
• evaluation of agronomic value for outstanding tetraploid selections,
• search for new sources of resistance to Potato leafroll virus (PLRV).

Laboratory of Biotechnology
Waldemar Marczewski, D.Sc.
phone: (+48 22) 729 92 48 ext. 215
e-mail: w.marczewski@ihar.edu.pl

Objectives:

• mapping of resistance genes to potato pathogens,
• detection of PCR-based markers for marker-assisted selection of potato and tomato,

RAPD markers linked to the locus PLRV.4 for resistance to Potato leafroll virus.

• in vitro collection of potato germplasm.

In vitro collection of diploid potato hybrids.

Laboratory for Research on resistance to Viruses

Joanna Sztangret-Wiśniewska, Ph.D.

phone: (+48 22) 729 92 48

e-mail: j.sztangret@ihar.edu.pl

Objectives:

• Characterization of strains of potato viruses,
• assassment of potato resistance to viruses,

Symptoms of infection PVYNTN

• evaluation of potato reaction to viral infection (PVY, PLRV, PVM, TRV),

Leaves necrosis on variety sensitive to PVY - grafting test

• resistance of potato breeding lines to viruses,
• collecting and maintenance of viral potato pathogens,
• evaluation of virus sources and test plants for detecting potato viruses,

Evaluation of PVYo on Chenopodium ssp. leaves

• studies on viruses causing necrotic symptoms in potato tubers (TRV, PMTV),
• advisory and training activities.

Laboratory of Potato Phytopathology
Renata Lebecka, Ph.D.
phone: (+48 22) 729 90 89 ext. 207

e-mail: r.lebecka@ihar.edu.pl

Objectives:

• monitoring of Phytophtora infestans population in Poland: characterization of P. infestans isolates for virulence, mating type, resistance to metalaxyl, mitochondrial DNA haplotype, genotype using SSR markers

Identification of mating type of Phytophthora infestans isolate.

• tests for potato resistance to Phytophthora infestans, Fusarium sulphureum
  and Erwinia carotovora subsp. atroseptica,

Potato tubers infected with Phytophthora infestans on 1-9 scale.

• new sources of resistance to Erwinia ssp.,

Potato tubers with soft rot symptoms.

• Marker Assisted Selection (MAS) of potato resistant to P.infestans possessing Rpi-phul gene
• analisis of polymorphism of molecular markers from potato chromosome V linked to the resistance to P. infestans and length of vegetation period in breeding material of potato in IHAR Młochów
• characterization of sources of resistance to P. infestans

Symptoms of infection of susceptible and resistant black nightshade (Solanum nigrum) to Phytophtora infestans.

• collection and maintenance of fungal and bacterial potato pathogens.

Colection of Phytophtora infestans.

Laboratory of Potato Virology
Associate Professor Jerzy Syller, D.Sc.
phone: (+48 22) 729 92 48 ext. 213
e-mail: j.syller@ihar.edu.pl

Objectives:

• studies on interactions between viruses, vectors and host plants in the process of infection and in the pathogenesis

Symptons of infection with PVYN on Chenopodium album	Extremely severe reaction of Physalis floridana plants to infection with PVYNW

• resistance of potato breeding lines to PLRV
• diagnostics of viruses and potato spindle tuber viroid (PSTVd) in breeding and experimental plant and tuber material

Disease symptoms on potato plants infected with PSTVd alone (left) and in co-infection with PLRV (right)