Organization structure
Department of Plant Transformation and Cell Engineering
Professor Wacław Orczyk, D.Sc. phone: (+48 22) 725 37 17 e-mail: w.orczyk@ihar.edu.pl www.ihar.edu.pl/ZIKiT
The complete information about Lab and Lab members, Research and Publications is under www.ihar.edu.pl/ZIKiT
Cell and Genetic Engineering Lab Wacław Orczyk, D.Sc. phone: (+48 22) 725 37 17; e-mail: w.orczyk@ihar.edu.pl
Lab members: Marta Dmochowska-Boguta M.Sc., Adam Kawałek M.Sc., Elżbieta Lasota M.Sc., Urszula Bojsza
Objectives:
- 1. Histopathological analysis of plant-pathogen interaction and elucidation of oxidative burst role in expression of plant defense against pathogen.
- 2. Identification of wheat transcripts induced / repressed upon infection of brown rust (Puccinia triticina).
- 3. Elaboration of experimental tools for functional gene analysis - adaptation of VIGS system for wheat functional genomics.
- 4. Application of transgenesis for analysis of gene function and for generation of wheat and barley plants with desired characteristics.
Genetic Transformation Lab Professor Anna Nadolska-Orczyk, D.Sc. phone: (+48 22) 725 36 11 ext. 284; e-mail: a.orczyk@ihar.edu.pl
Lab members: Agnieszka Bińka M.Sc., Sebastian Gasparis M.Sc., Wojciech Zalewski M.Sc., Marta Mikucka M.Sc., Małgorzata Wojciechowska
Our research is focused on application of genetic transformation of cereal (wheat, triticale, oat) and pea cultivars via Agrobacterium tumefaciens for analysis of gene function and obtaining breeding lines with improved characteristics.
Objectives:
- 1. Agrobacterium-mediated transformation of cereals: wheat (Triticum aestivum L.), triticale (Triticale), oat (Avena sativa L.) and barley (Hordeum vulgare L.):
- • adaptation of genetic transformation methods developed in the lab to polish breeding lines and cultivars,
- • analysis of factors influencing expression and silencing of transgenes in the listed species.
- 2. Application of RNAi in functional genetics and biotechnology of cereals and pea:
- • analysis of influence of RNAi process on the level of expression and silencing of native genes in wheat, triticale, barley and pea,
- • silencing of Pina i Pinb genes in wheat and triticale by RNAi method and analysis of grain texture,
- • functional analysis of CKX gene family in regulation of cytokinin level in barley and wheat and its influence on phenotype modification.
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