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Department of Plant Biotechnology and Cytogenetics Department of Plant Biotechnology and Cytogenetics
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Department of Plant Transformation and Cell Engineering Department of Plant Transformation and Cell Engineering
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Organization structure

Department of Plant Transformation and Cell Engineering

Wacław Orczyk, D.Sc.
phone: (+48 22) 725 37 17
e-mail: w.orczyk@ihar.edu.pl
www.ihar.edu.pl/ZIKiT

The complete information about Lab and Lab members, Research and Publications is under Plant Transformation and Cell Engineering Lab


Genetic Transformation Lab
Anna Nadolska-Orczyk, D.Sc.
phone: (+48 22) 725 36 11 ext. 284;
e-mail: a.orczyk@ihar.edu.pl

Lab members: Małgorzata Wiweger Ph.D., Agnieszka Bińka M.Sc., Agnieszka Karaś M.Sc., Halina Milczarek, Sebastian Gasparis

Our research is focused on genetic transformation of cereal (wheat, triticale, oat) and pea cultivars via Agrobacterium tumefaciens and analysis of factors responsible for expression and silencing of transgenes/native genes.

 

Objectives:

  • Agrobacterium-mediated transformation of cereals: wheat (Triticum aestivum L.), triticale (Triticale), oat (Avena sativa L.) and barley (Hordeum vulgare L.):
    - analysis of expression/silencing of transgenes,
    - analisys of RNAi processes influencing level of transgene expression/silencing in wheat and triticale,
    - development of in planta transformation method,
    - silencing selected native genes of cereals: pinA and pinB genes,
    - functional analysis of the genes and obtaining plants with desired characteristics.
  • Agrobacterium-mediated transformation of pea (Pisum sativum L.):
    - investigation of RNAi processes of genes coding mitochondrial proteins in pea.

 


Cell and Genetic Engineering Lab
Wacław Orczyk, D.Sc.
phone: (+48 22) 725 37 17;
e-mail: w.orczyk@ihar.edu.pl

Lab members: Urszula Piskurewicz M.Sc., Beata Olejnik M.Sc., Ilona Mirowska M.Sc., Dominik Kuć M.Sc., Teresa Bracik

 

Objectives:

  • isolation and functional analysis of plant transcripts/genes involved in plant-pathogen interaction:
    - histopathological analysis of plant – pathogen interaction,
    - identification of wheat transcripts induced upon rust (P. triticina) infection,
    - elaboration of experimental tools for functional analysis,
    - adaptation of VIGS system for wheat functional genomics,
    - application of transgenesis for functional analysis of isolated transcripts/genes and for genetic modification.