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Department of Plant Biotechnology and Cytogenetics Department of Plant Biotechnology and Cytogenetics
Department of Plant Physiology and Biochemistry Department of Plant Physiology and Biochemistry
Department of Plant Pathology Department of Plant Pathology
Department of Plant Breeding and Genetics Department of Plant Breeding and Genetics
Department of Plant Transformation and Cell Engineering Department of Plant Transformation and Cell Engineering
Department of Seed Science and Technology Department of Seed Science and Technology
National Centre for Plant Genetic Resources National Centre for Plant Genetic Resources
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Organization structure

Department of Plant Transformation and Cell Engineering

Professor Wacław Orczyk, D.Sc.
phone: (+48 22) 725 37 17
e-mail: w.orczyk@ihar.edu.pl
www.ihar.edu.pl/ZIKiT

The complete information about Lab and Lab members, Research and Publications is under www.ihar.edu.pl/ZIKiT

 

 

Cell and Genetic Engineering Lab
Wacław Orczyk, D.Sc.
phone: (+48 22) 725 37 17;
e-mail: w.orczyk@ihar.edu.pl

Lab members: Marta Dmochowska-Boguta M.Sc., Adam Kawałek M.Sc., Elżbieta Lasota M.Sc., Urszula Bojsza

 

Objectives:

  • 1. Histopathological analysis of plant-pathogen interaction and elucidation of oxidative burst role in expression of plant defense against pathogen.
  • 2. Identification of wheat transcripts induced / repressed upon infection of brown rust (Puccinia triticina).
  • 3. Elaboration of experimental tools for functional gene analysis - adaptation of VIGS system for wheat functional genomics.
  • 4. Application of transgenesis for analysis of gene function and for generation of wheat and barley plants with desired characteristics.

 

 


Genetic Transformation Lab
Professor Anna Nadolska-Orczyk, D.Sc.
phone: (+48 22) 725 36 11 ext. 284;
e-mail: a.orczyk@ihar.edu.pl

Lab members: Agnieszka Bińka M.Sc., Sebastian Gasparis M.Sc., Wojciech Zalewski M.Sc., Marta Mikucka M.Sc., Małgorzata Wojciechowska

Our research is focused on application of genetic transformation of cereal (wheat, triticale, oat) and pea cultivars via Agrobacterium tumefaciens for analysis of gene function and obtaining breeding lines with improved characteristics.

 

Objectives:

  • 1. Agrobacterium-mediated transformation of cereals: wheat (Triticum aestivum L.), triticale (Triticale), oat (Avena sativa L.) and barley (Hordeum vulgare L.):
  • • adaptation of genetic transformation methods developed in the lab to polish breeding lines and cultivars,
  • • analysis of factors influencing expression and silencing of transgenes in the listed species.
  • 2. Application of RNAi in functional genetics and biotechnology of cereals and pea:
  • • analysis of influence of RNAi process on the level of expression and silencing of native genes in wheat, triticale, barley and pea,
  • • silencing of Pina i Pinb genes in wheat and triticale by RNAi method and analysis of grain texture,
  • • functional analysis of CKX gene family in regulation of cytokinin level in barley and wheat and its influence on phenotype modification.