Organization structure

Department of Plant Biotechnology and Cytogenetics

Professor Janusz Zimny, D.Sc.
phone: (+48 22) 725 22 52
e-mail: j.zimny@ihar.edu.pl

Laboratory of Plant Tissue Culture
Professor Janusz Zimny, D. Sc.
phone: (+48 22) 725 22 52
e-mail: j.zimny@ihar.edu.pl

Objectives:
In vitro culture

• experimental embryology and somatic embryogenesis - plant regeneration by induction of somatic embryos from different explants, effect of light spectral properties on somatic embryogenesis,
• androgenesis - regeneration of DH plants and homozygous lines from isolated microspores and anther culture (wheat, rye, triticale, and barley) for breeding progress
• cryopreservation in in vitro cultures for Gen Bank conservation,
• transgenic plants, plant tissue transformation for research study and industrial use (particle gun, Agrobacterium, PEG),
• RAPDs and numerical taxonomy - analysis of genetic distance and diversity to establish the stability of lines and their relationship.

Biosafety activity www.ihar.edu.pl/gene_bank/GMO

• creation of GMP diagnostic laboratory for quantitative and qualitative GMO detection,
• rising public awareness on GMP as a member of International "GMP-Network",
• supporting the GMO law implementation in Poland,
• contribution to the National Biosafety Framework of Poland,
• gene expression study.
  

Laboratory of Cytogenetics
Jerzy Macewicz, D.Sc.
phone: (+48 22) 725 36 11 ext. 338
e-mail: j.macewicz@ihar.edu.pl

Objectives:
Variation in the 3n subaleurone protein tissue of barley

• protein content in doubled haploids and diploid lines,
• inter- and intra- spike variation in the 3n subaleurone protein tissue,
• study of the inter- and intra spike variation of protein content in the 3n subaleurone layer,
• histochemical test for protein quantity assessment in single seeds,
• inheritance of subaleurone proteins in selection for brewery types of barley,
• the effect of endosperm protein content and phenotypic characteristics of sublines.

Biomechanics of crop plants

• mechanical properties of stem in cereals and pea in relation to lodging resistance,
• heritability and genetic markers of stem mechanical traits,
• fracture properties of seeds in relation to technological quality,
• photomorphogenesis and plant biomechanics and architecture.

Role of cell wall and cell wall proteins in control of plant growth

• acid growth, cell wall rheology in relation to shoot elongation rate,
• expansin expression and activity,
• photomodulation of growth rate and cell wall proteins expression,
• cell wall modifications in low temperature hardening of rape plants.

Cellulose biosynthesis

• expression of cellulose synthase subunits,
• photocontrol of cellulose synthesis,
• cell wall structural mutations.

Morphological traits of the ear and the grain yield

• morphogenesis of the ear, spikelets and florets in cereals,
• effect of duration of particular ear developmental phases on grain number,
• ear morphogenesis analysis for in in vitro microspore and anther cultures.
  
  
  
  

Laboratory of Genetically Modified Organisms (GMO)

Sławomir Sowa, Ph.D.
phone: (+48 22) 725 36 11 ext. 238
e-mail: s.sowa@ihar.edu.pl

Objectives:

• creation of GMP diagnostic laboratory for quantitative and qualitative GMO detection,
• rising public awareness on GMP as a member of International “GMP–Network”,
• supporting the GMO law implementation in Poland,
• contribution to the National Biosafety Framework of Poland.