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Bonin Research Center

Agnieszka Przewodowska, PhD
76-009 Bonin k/ Koszalina
tel. (+48) 94 342 30 31 lub +48) 94 342 69 97
fax. (+48) 94 342 70 28
e-mail: a.przewodowska@ihar.edu.pl
strona www: www.ziemniak-bonin.pl

Department of Potato Protection and Seed Science at Bonin
more information www.ziemniak-bonin.pl

Laboratory of Seed Potato (more...)

Tomasz Erlichowski, PhD
phone (+48 94) 342 30 31
e-mail: wrobel@ziemniak-bonin.pl

Laboratory of Potato Protection (more...)

Professor Józefa Kapsa, D.Sc.
phone +48 94 342 30 31 w. 229
 e-mail: kapsa@ziemniak-bonin.pl

Laboratory of Molecular Diagnostics and Biochemistry (more...)

Krzysztof Treder, Ph.D.
phone +48 94 342 30 31 w. 207
e-mail: k.treder@ihar.edu.pl

Laboratory of Potato Gene Resources and Tissue Culture (more...)

Agnieszka Przewodowska, PhD
phone +48 94 342 30 31 w. 213
 e-mail: sekrecka@ziemniak-bonin.pl

Laboratory of Market Research (more...)

vacat

 

Edition of "Ziemniak Polski"

Publisher of Department of Potato Protection and seed Science at Bonin
 Quartarly Journal - ISSN 1425-4263

 Editorial Board Chairman: Janusz Urbanowicz, Ph.D.

 Managing Editor: Krystyna Żalejko, M.Sc.
 phone +48 94 342 30 31 w.205
 e-mail: zalejko@ziemniak-bonin.pl

 Papers are published only in Polish.


Laboratory of Seed Potato (top...)

Tomasz Erlichowski, PhD
phone (+48 94) 342 30 31
e-mail: wrobel@ziemniak-bonin.p

Objetives:

  • aphid monitoring in seed potato crops,
  • epidemiology of potato viruses, forecasting the incidence of potato viruses, control of viruses` transmission in the field, timing of aphid control and haulm desiccation,
  • studies on influence of plantvirus-environment interaqction with regard to epidemiology and control,
  • improvement of seed potato production system, the characterization of suitability of different cultivars for definite technology,
  • improvement of methods used for health inspection and qualification of seed potatoes.

 


Laboratory of Potato Protection (top...)

Józefa Kapsa, D.Sc., Assoc. Prof. IHAR-PIB
phone (+48 94) 342 30 31 w. 229
e-mail: kapsa@ziemniak-bonin.pl

 

Objectives:

  • improvement of methods for assessing potato variety resistance to diseases,
  • epidemiological studies of potato pathogens with special references to Phythophtora infestans, Alternaria, Rhizoctonia, Erwina and tuber disease pathogens,
  • biology and ecology of potato pests, e.g. Lepitonotarsa decemlineata Say., Elateridae and Scarabeidae,
  • evaluation of colorado potato beetle preference to different potato varieties useful for organic agriculture,
  • studies on potato varietal reaction on herbicides,
  • studies on influence of plant-pathogen/pest-environment interaction on variability of potato agrophages,
  • improvement of methods for pathogen diagnostic,
  • improvemnt of startegies for potato pathogens control (infection sources, monitoring net of Phythophtora infestans and  Alternaria in Poland, forecasting, decision support system (DSSs),
  • risk assessment of pathogen/past resistance development to pesticides,
  • development of environmental friendly elements into integrated protection of potato crops against agrophages,
  • evaluation of new products for chemical potato protection (fungicides, herbicides, insecticides, desiccants, seed dressers) for registration in Poland,
  • collection of fungal and bacterial potato pathogens.

 


Laboratory of Molecular Diagnostics and Biochemistry (top...)

Krzysztof Treder, Ph.D.
phone (+48 94) 342 30 31 w. 207
e-mail: k.treder@ihar.edu.pl

 

Scientific staff:

Krzysztof Treder, Ph.D.  - assistant professor
phone: (+48 94) 342 30 31 w. 207
e-mail: k.treder@ihar.edu.pl

Włodzimierz Przewodowski, Ph.D. - assistant professor
phone: (+48 94) 342 30 31 w. 211
e-mail: w.przewodowski@ihar.edu.pl

Research and Technical staff:

Anna Pawłowska, M.Sc.,
Bogumiła Zacharzewska, M.Sc.,
Wioleta Stochła, M.Sc.,
Mateusz Mielczarek, M. Sc.,
Maria Fedczak, senior technician
tel.: (+48 94) 342 30 31 w. 241

Laboratory description:

In the Laboratory of Molecular Diagnostics and Biochemistry are developed immunological and molecular methods to detect the most important potato viruses and bacteria. Polyclonal antibodies are produced in rabbits for the detection of potato viruses (PVY, PVM, PLRV, PVX, PVS), and for detection of Clavibacter michiganensis ssp. sepedonicus (Cms). Molecular diagnostics detection methods based on PCR as well as methods based on isothermal amplification of nucleic acids are developed and optimized. Another line of research focuses on potato proteins having potential biotechnological properties, including peroxidases, proteins and peptides with antibiotic activity, and enzymes involved in response to biotic and abiotic stress. Protein and molecular markers are also studied in the context of pathogens strains and potato varieties differentiation. In 2007 procedure of isolation of peroxidases from potato pulp was patented and in recent years seven patents (including one international) concerning the diagnosis of bacterial Cms were obtained. In addition, the laboratory is conducting student internships for final year students from faculties of biological and biotechnological profiles.

Laboratory description:

  • Development of methods for detection of potato viruses (PVY, PVM, PLRV, PVX, PVS) and Potato spindle tuber viroid (PSTVd) by RT-PCR and isothermal nucleic acid amplification.
Comparison of detection sensitivity of potato virus Y (PVY) by isothermal assay RT-LAMP to classical RT-PCR (A) and real‑time RT-PCR (B).
  • The development of sensitive and specific tests for the detection of Clavibacter michiganensis ssp. sepedonicus.
Colonies of strains of Clavibacter michiganensis ssp. sepedonicus (Cms) on nutrition plates (A).  Specificity of developed lateral flow devices to different strains of Cms (B). Cms cells immobilized on immunosorbent surface,  magnified 2500‑fold using scanning elektron microscope (C).
  • Preparation of nanomaterials including organic polymers, colloids of metals (gold, silver, platinum and copper) as well as magnetic microspheres and their use in the detection of potato pathogens.
Nanoparticles of colloidal gold (20-30 nm) prepared by reduction of chloroauric acid (A). Silica microspheres (400-500 nm) prepared from tetraetoxysilane (TEOS) in ammoniated methanol (B). Polyaniline suspension prepared by free-radical polymerization in acid environment (C). Magnetic particles prepared by co-precipitating ferric and ferrous salts in an alkaline solution before (I) and after collecting on the magnet (II). Magnet (III) without a tube (D). Detection of  PLRV by ELISA using magnetic particles coated with antibodies specific to the virus (E).
  • The use of potato industry wastes (pulp, water) to obtain valuable proteins, including peroxidases, protein protease inhibitors, and proteins having antibiotic activity with respect to microbial pathogens of plants and humans.
The molecular heterogeneity of peroxidases extracted from potato pulp (from 2.5 to 10g). Electrophoresis was carried out in a polyacrylamide gel under native conditions. Activity stained with 3,3 ', 5,5'-tetramethylbenzidine (A). The effectiveness of the removal of phenols from water by peroxidase from potato pulp. The initial amount of phenol - 100% (B).
  • Methods of identification of potato varieties based on electrophoretic profile of potato tuber proteins and DNA profile using standards of potato varieties from the collection kept in the Potato Gene Bank.
Identification of potato cultivars by native polyacrylamide electrophoresis. Harp – standard of the Harpun cultivar, Pas – standard of the Pasat cultivar. 1-7 – potatoes identified as cultivar Harpun. M – markers of molecular weight.
  • Exploration and characterization of enzymes whose activity may be a marker of tolerance of potato varieties to biotic and abiotic stress.
  • Research on limiting the spread of bacterial and fungal pathogens of potato by developing methods for disinfecting surfaces of tubers, containers, walls and agricultural equipment.

 

Publications 2010-2017:

  • Stochła W., Przewodowski W., Przewodowska A., Salamońska K. 2017. Immunodiagnostyczne metody wykrywania i identyfikacji bakteryjnych patogenów ziemniaka. Ziemniak Polski 1: 14-20.
  • Szarek D. Przewodowska A. 2017. Patatyna – Białko ziemniaka o cennych właściwościach. Ziemniak Polski 1: 32-36
  • Kurnik K., Krzyżyński M., Treder K., Tretyn A., Tyburski J. 2017. Study on utilizing solid food industry waste with brewers’ spent grain and potato pulp as possible peroxidase sources. J. Food Biochem. doi:10.1111/jfbc.12446.
  • Treder K., Zacharzewska B. 2017. Opracowanie nowych starterów do wykrywania wirusa M ziemniaka (Potato virus M, PVM) za pomocą testu RT-PCR. Ziemniak Polski, 3: 32-35.
  • Wróbel S., Kęsy J., Treder K. 2017. Effect of growth regulators and ethanol on termination of dormancy in potato tubers. Am. J. Potato Res. doi:10.1007/s12230-017-9592-2.
  • Kurnik K., Treder K., Twarużek M., Grajewski A., Tretyn A., Tyburski J. 2017. Potato pulp as the peroxidase source for 2,4-dichlorophenol removal. Waste Biomass Valor. doi:10.1007/s12649-017-9863-7.
  • Przewodowski W, Przewodowska A. 2017. Development of a sensitive and specific polyclonal antibody for serological detection of Clavibacter michiganensis subsp. sepedonicus. PLoS ONE 12(1):e0169785.doi:10.1371/journal.pone.0169785.
  • Gryń G, Pastuszewska T., Przewodowski W. 2016. Ocena patogeniczności wybranych szczepów Clavibacter michiganensis subsp. sepedonicus pochodzących z kolekcji NCPPB w Wielkiej Brytanii. Progress in Plant Protection / Postępy w Ochronie Roślin  56: 73-78.
  • Treder K., Pawłowska A., Chołuj J. 2016. Identyfikacja odmian ziemniaka za pomocą elektroforezy natywnej białek i molekularnego testu ISAP. Ziemniak Polski, 4: 21-27.
  • Treder K. 2015. Metody wykrywania obecności wirusów ziemniaka. Ziemniak Polski, 4: 18-23.
  • Przewodowska A., Zacharzewska B., Chołuj J., Treder K. 2015. A one-step, real-time reverse transcription loop-mediated isothermal amplification assay to detect potato virus Y. Am. J. Potato. Res. 92: 303-311.
  • Kurnik K., Treder K., Skorupa-Kłaput M., Tretyn A.,Tyburski J. 2015. Removal of phenol from synthetic and industrial waste water by potato pulp peroxidases. Water Air Soil Pollut. 2015; 226(8): 254. doi:  10.1007/s11270-015-2517-0.
  • Treder K., Zacharzewska B., Przewodowska A., Przewodowski W., Otulak K. 2015. Ion-exchange membrane chromatography as an alternative method of separation of potato Y virus. Plant Breeding and Seed Science 72: 56-67.
  • Wróbel S., Kęsy J., Treder K. 2015. Effect of ethanol and plant growth regulators on termination of potato microtuber dormancy. Plant Breeding and Seed Science 71: 23-36.
  • Przewodowski W., Chołuj J., Przewodowska A. 2015. Wpływ różnych sposobów izolacji kwasów nukleinowych z bakterii na czułość testu PCR. Progress in Plant Protection / Postępy w Ochronie Roślin 55: 321-326. DOI: 10.14199/ppp-2015-056.
  • Stochła W., Przewodowska A., Przewodowski W. 2015. Przydatność przeciwciał króliczych uzyskiwanych dwiema metodami do wykrywania Clavibacter michiganensis subsp. sepedonicus testem DAS-ELISA. Progress in Plant Protection / Postępy w Ochronie Roślin 55: 352-357.
  • Pietraszko M., Gryń G., Pastuszewska T., Przewodowski W., Przewodowska A. 2015. Podatność wybranych odmian ziemniaka na porażenie bakteriami Clavibacter michiganensis subsp.sepedonicus w zróżnicowanych warunkach glebowych. Biuletyn IHAR 277: 99-108.
  • Stochła W., Przewodowska A., Przewodowski W. 2015. Wykrywanie bakterii Clavibacter michiganensis subsp. sepedonicus przy użyciu przeciwciał króliczych uzyskiwanych dwoma różnymi sposobami. Prog. Plant Prot. 55, 3: 352-357.
  • Przewodowski W., Chołuj J., Przewodowska A. 2015. Wpływ różnych sposobów izolacji kwasów nukleinowych z bakterii na czułość testu  PCR. Prog. Plant Prot. 55, 3: 321-326.
  • Przewodowska A., Zacharzewska B., Chołuj J., Treder K. 2015. A one-step, real-time reverse transcription loop-mediated isothermal amplification assay to detect Potato virus Y. American Journal of Potato Research. 92: 303-311.
  • Zacharzewska B., Przewodowska A., Treder K. 2014. The adaptation of silica capture RT-PCR for the detection of potato virus Y. American Journal of Potato Research, 91: 525-531.
  • Chołuj J., Przewodowski W. 2014. Technika PCR i jej modyfikacje w identyfikacji patogenów ziemniaka. Ziemniak Polski, 24(3): 40-45.
  • Pawłowska A., Treder K. 2014. Peroksydazy-małe enzymy o wielkim znaczeniu. Ziemniak Polski, 24(1).
  • Zacharzewska B., Treder K. 2014. Test ELISA jego modyfikacje. Ziemniak Polski, 24(1).
  • Treder K., Przewodowska A. 2013. Application of RT-LAMP for direct detection of potato viruses in dormant tubers. Acta Phytopathologica Sinica, 43: 448. Streszczenie posteru.
  • Przewodowska A, Przewodowski W., Treder K. 2013. Ion-exchange membrane chromatography – a new tool for plant virus purification. Acta Phytopathologica Sinica, 43: 448-449. Streszczenie posteru.
  • Przewodowski W., Przewodowska A. and Treder K. 2013. Application of colloidal metal nanoparticles in diagnosis of quarantine bacteria – Clavibacter michiganensis subsp. sepedonicus. Abstract 10th International Congress of Plant Pathology. Acta Phytopathologica Sinica 43: 370. Streszczenie posteru.
  • Przewodowski W., Przewodowska A. 2012. Wykorzystanie biochemicznych i molekularnych metod w diagnostyce patogenów oraz identyfikacji jednorodności odmianowej ziemniaka. s. 57-66. W: „Produkcja i rynek ziemniaka” (J. Chotkowski, red.). Wydawnictwo Wieś Jutra, Warszawa, 340.
  • Przewodowska A., Treder K. 2012. Purification of plant viruses by membrane ion-exchange chromatography. New Biotechnology, 29: S133. Streszczenie posteru.
  • Waleron M., Waleron K., Kamasa J., Przewodowski W., Lojkowska E. 2011. Polymorphism analysis of housekeeping genes for identification and differentiation of Clavibacter michiganensis subspecies. Eur. J. Plant Pathol. 131:341–354.
  • Pilecki T., Treder K. 2010. Wpływ okresu przechowywania ziemniaków na wykrywalność wirusów PLRV, PVM oraz PVY w bulwach. Prog. Plant Prot./Post. Ochr. Roślin, 50: 723–727.
  • Przewodowski W. 2010. Antimicrobial influence of colloidal nanoparticles on quarantine pathogen Clavibacter michiganensis ssp. sepedonicus. New Biotechnology - Abstracts of the 15th European Congress on Biotechnology 29: 132 Streszczenie posteru.
  • Przewodowski W., Barnyk A. 2010. Nowe metody diagnostyczne do wykrywanie fitopatogennych bakterii ziemniaka. Ziemniak Polski, 1: 1-4.

Patents:

  • Patent US 12/676 671 . “Immunological tests for the presence of bacteria which make use of antibodies obtained using a specific method” Granted  17.09.2013r. by Unated States Patent Office (USPTO). (Base – international patent proposal PCT/PL2008/050015 WIPO-Geneve). Inventor: Dr eng. Włodzimierz Przewodowski.
  • Patent P 383366. Zestaw do wykrywania albo przeżyciowego izolowania bakterii Clavibacter michiganensis ssp. sepedonicus w analizowanej próbce. Granted 12.09.2012r by Republic of Poland Patent Office (UP RP). Inventor: Dr eng. Włodzimierz Przewodowski.
  • Patent P 383365. Zestaw inkubacyjny do wykrywania albo przeżyciowego izolowania wybranych bakterii, w szczególności Clavibacter michiganensis ssp. sepedonicus. Granted 12.09.2012r by UP RP. Inventor: Dr eng. Włodzimierz Przewodowski.
  • Patent P 383364. Sposób wykrywania obecności bakterii Clavibacter michiganensis ssp. sepedonicus z wykorzystaniem membran poliwęglanowych zawierających immobilizowane przeciwciała. Granted 12.09.2012r by UP RP. Inventor: Dr eng.  Włodzimierz Przewodowski.
  • Patent P 383363. Sposób wykrywania obecności bakterii Clavibacter michiganensis ssp. sepedonicus w analizowanej próbce. Granted 12.09.2012r by UP RP. Inventor: Dr eng. Włodzimierz Przewodowski.
  • Patent P 383361. Test immunologiczny na obecność bakterii. Granted 25.03.2011r. by UP RP. Inventor: Dr eng. Włodzimierz Przewodowski.
  • Patent P 383362. Sposób usuwania śluzów bakteryjnych. Granted 24.03.2011r. by UP RP. Inventors: Dr eng. Włodzimierz Przewodowski, Prof. Jerzy Lewosz
  • Patent PL 195200 B1. Sposób otrzymywania peroksydazy z wycierki ziemniaczanej. Granted 31.08.2007r. by UP RP. Inventor: Prof. Jerzy Lewosz.

 


Laboratory of Potato Gene Resources and Tissue Culture (top...)

Agnieszka Przewodowska, PhD
phone (+48 94) 342 30 31 w. 213
e-mail: danka.sekrecka@wp.pl

 

Objectives:

  • maintenance of the potato genetic resources in vitro, cost effecinecy studies,
  • micro and mini tubers production and improvement of in vitro production methods,
  • valorisation of collected materials.

 


Laboratory of Market Research (top...)

vacat.

Objectives:

  • integration of agricultural markets,
  • futures markets for agricultural commodities,
  • economic risk in agriculture,
  • seed potato market analysis,
  • analysis of domestic and international potato market (supply-demand analysis, prices, marketing effectiveness, organizational models) in comparison with other agricultural markets,
  • analysis of potato production costs, effectiveness, economical and organizational valuation and processing technologies.
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