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Department of Plant Pathology

Professor Edward Arseniuk, D.Sc.
phone: +48 22 733 46 30
e-mail: e.arseniuk@ihar.edu.pl

Laboratory of Breeding for Disease Resistance (more...)

Professor Edward Arseniuk, D.Sc.
phone: +48 22 733 46 30
e-mail: e.arseniuk@ihar.edu.pl

Laboratory of Plant Diseases (more...)

Piotr Ochodzki, Ph.D.
phone: (+48 22) 733 46 37; Mobile: 603 454 784
e-mail: p.ochodzki@ihar.edu.pl

Laboratory of Quarantine Organisms (more...)

Professor Edward Arseniuk, D.Sc.
phone: +48 22 733 46 30
e-mail: e.arseniuk@ihar.edu.pl

 


Laboratory of Breeding for Disease Resistance

Paweł Czembor, D.Sc., Assoc. Prof. IHAR-PIB
phone: (+48 22) 733 46 32
e-mail: p.czembor@ihar.edu.pl

Objectives:

  • development of breeding lines resistant to main cereal diseases using molecular selection methods and in vitro culture techniques,
  • search for new sources of resistance and identification molecular markers linked to resistance in wheat, triticale and barley to necrotrophic and biotrophic fungi,
  • developing molecular markers linked to maiting types and pathogenicity of Phaeosphaeria (Stagonospora, syn. Parastagonospora) nodorum i Mycosphaerella graminicola (Septoria tritici, syn. Zymoseptoria tritici),
  • molecular studies of genetic variation in population of pathogenic fungi
  • monitor, collect and characterize cereal rust (Puccinia triticina and P. striiformis) and septoria pathogen populations (Parastagonospora nodorum and Zymoseptoria tritici) for pathogenicity.

Genotypic variation in morphology and resistance to septoria leaf and glume blotch among doubled haploid lines developed through anther culture from Pinokio × Bogo F1 generation.

 


Laboratory of Plant Disease

Piotr Ochodzki, Ph.D.
phone: (+48 22) 733 46 37; Mobile: 603 454 784
e-mail: p.ochodzki@ihar.edu.pl

Objectives:

  • breeding of wheat, triticale and durum wheat genotypes with increased resistance to diseases caused by Fusarium spp.
  • evaluation of breeding materials and the search for sources of resistance of triticale, wheat and durum wheat towards diseases caused by Fusarium spp.
  • improving of testing methodology of cereals and corn resistance to Fusarium head blight (FHB) and Fusarium ear rot,
  • assessment of wild relatives of cereals in order to identify possibilities for use in resistance breeding,
  • research on the role of mycotoxins in the pathogenesis of diseases of cereals caused by Fusarium spp.
  • analysis of population variation of fungi Fusarium spp. on Polish territory,
  • characterization of isolates of Fusarium spp. for the production of mycotoxins,
  • analysis of contents of selected mycotoxins in cereals and corn,
  • determination of ergosterol in the plant material,
  • improvement of methods of mycotoxins analysis,
  • assessment of the mycotoxins produced by endophytic fungi in grasses,
  • analysis of  the content of ergot alkaloids in cereal grains,
  • characterization of proteinaceous toxins produced by Stagonospora nodorum
  • qualitative and quantitative analysis of mono- di- and polysaccharides in plant material,
  • research on the cultivation of maize and triticale in terms of organic farming.

 


Laboratory of Quarantine Organisms

Professor Edward Arseniuk, D.Sc.
phone: +48 22 733 46 30
e-mail: e.arseniuk@ihar.edu.pl

Objectives:

  • Disease resistance testing to potato wart disease.

Resistance to S. endobioticum is important to maintain crop health. Growers need reliable information about resistances, to different pathotypes of S. endobioticum, when they have to choose a variety. Laboratory of Quarantine Organisms runs different types of tests, according to European and Mediterranean Plant Protection Organization (EPPO) standards, and keeps the most relevant pathotypes od S. endobioticum and potato cultivars of differential set.

For some specific crops, information about resistance to different pathotypes of S. endobioticum must be included in the variety description that is required to define and protect a variety. The official tests are curried out using the Glynne-Lemmerzahl method, according to EPPO Standard PM 7/28 published by the European and Mediterranean Plant Protection Organization. For resistant cultivars of potato to different pathotypes of S. endobioticum an appropriate Certificates of resistance are issued.

Inoculation of potato sprouts using The Glynne-Lemmerzahl method.
Reaction of susceptible potato genotype to pathotype 1(D1) 4 weeks after inoculation.
  • Monitoring and identification ofS. endobioticum isolates.

Individual isolates of S. endobioticum may be able to overcome specific resistances. It is therefore useful to define pathotypes based on their reaction to a range of well-characterized differential cultivars. Pathotests are curried out using the Glynne-Lemmerzahl method, according to EPPO Standard PM 7/28, on at least 20 differential cultivars of potato (if it necessary) to include less known pathotypes.

Different reaction of cv. Asche Sämling to pathotype 3(M1) and 2(Ch1) of S. endobioticum.
  • Collection of S. endobioticum pathotypes and isolates >

Laboratory has an pathotype and isolate collection of S. endobioticum. The collection is used for resistance testing in connection with cultivar registration trials. Different pathotypes and the isolates are validated regularly through biotests on differentiating resistant cultivars. The collection contains the most relevant pathotypes of S. endobioticum from Western Europe : 1(D1), 2(G1), 6(O1), 8(F1), 18(T1); from Poland: 2(Ch1), 3(M1), 39(P1) as well as the isolates from The United Kingdom, Sweden, Bulgaria, Denmark, Greece and Poland.

Multiplication of potato wart disease on tubers of susceptible cultivars.
  • Monitoring of Globodera rostochiensis and G. pallida population changes in Poland. Identification of PCN pathotypes in soil samples.

  • Collection and maintance of five pathotypes of golden potato cyst nematodes (G. rostochiensis Ro1-Ro5) and three pathotypes of white potato cyst nematodes (G. pallida Pa1-Pa3).
  • Assessment of resistance of potato variety and breeding lines to G. rostochiensis (Ro1-Ro5) and G. pallida (Pa1-Pa3) pathotypes.
  • Detection of Ralstonia solanacearum in potato tubers, waste water and solid wastes.

Bacterial ooze from cut tomato stem infected with Ralstonia solanacearum.

Detection of Ralstonia solanacearum using immunofluorescence methods.


Virulent colonies of Ralstonia solanacearum on TZC medium.

Effect of Ralstonia solanacearum infection on potato plant.
  • Detection and diversity of Clavibacter michiganensis subsp. sepedonicusisolates using molecular techniques.

Clavibacter michiganensis subsp. sepedonicus causes a dangerous bacterial disease called ring rot of potato. The disease is of quarantine status in the EU as a result of losses it causes easiness of its spread. It means, this disease is controlled by national regulations and there is zero tolerance for this pathogen in the potato crops. This bacterium generates a huge threat for seed material and breeding production and hinders trading of potato in the EU and other countries. Lack of sufficiently sensitive methods of detection and identification is the reason for poor elimination of this pathogen.

Transverse section of potato tuber infected by ring rot.

 

Publications 2010 – 2015:

  • Flath K., Przetakiewicz J., van Rijswick, P.C.J., Ristau, V., and van Leeuwen G.C.M. 2014. Interlaboratory tests for resistance to Synchytrium endobioticum in potato by the Glynne-Lemmerzahl method. OEPP/EPPO Bull. 44(3): 1-8.
  • Gawińska-Urbanowicz H., Michalak K., Przetakiewicz A., Przetakiewicz J., Śliwka J., Sobkowiak S., Węgierek A., Yin Z. 2014. Gromadzenie, charakterystyka w zakresie biologii oraz przechowywanie ras i patotypów najważniejszych patogenów ziemniaka. Monografie i rozprawy naukowe IHAR-PIB-2014 nr 48, str. 120-126.
  • Jakuczun H., Przetakiewicz J., Wasilewicz-Flis I., Hara-Skrzypiec A., Smyda P., Zimnoch-Guzowska E. 2013. Mieszańce diploidalne ziemniaka źródłem odporności na raka ziemniaka (Synchytrium endobioticum (Schilb.) Perc.). Biuletyn IHAR 268: 113-119.
  • Milczarek D., Flis B. Przetakiewicz A, 2011. Suitability of molecular markers for selection of potatoes resistant to Globodera spp. Am. J. Pot. Res. 88:245–255.
  • Milczarek D., Przetakiewicz A., Kamiński P., Flis B. 2014. Early selection of potato clones with the H1 resistance gene - the relation of nematode resistance to quality characteristics. Czech J. Genet. Plant Breed., 50(4): 278-284.
  • Pastuszewska T., Gryń G., Węgierek A., Przetakiewicz A. 2014. Śledzenie zmian patogeniczności w populacjach Clavibacter michiganensis ssp. sepedonicus - sprawcy bakteriozy pierścieniowej ziemniaka oraz Ralstonia solanacearum - sprawcy śluzaka ziemniaka. Monografie i rozprawy naukowe IHAR-PIB-2014 nr 48, str. 249-257.
  • Przetakiewicz A. 2006. Ralstonia solanacearum – sprawca śluzaka roślin psiankowatych. Biuletyn IHAR 242: 195-203.
  • Przetakiewicz A. 2010. Ocena odporności wybranych polskich odmian ziemniaka na mątwika ziemniaczanego (Globodera rostochiensis) i mątwika agresywnego (G. pallida). Ziemniak Polski 1: 39-42.
  • Przetakiewicz A. 2011. Monitoring odporności linii hodowlanych ziemniaka na patotypy mątwika ziemniaczanego i agresywnego w latach 2007-2010. Ziemniak Polski 3: 12-15.
  • Przetakiewicz A. 2013. The first report of Globodera rostochiensis pathotypes Ro5 occurrence in Poland. Plant Disease vol. 97, 8:1125.
  • Przetakiewicz A. 2014. Śledzenie zmian w populacjach nicieni Globodera rostochiensis i G. pallida - kwarantannowych szkodników ziemniaka. Monografie i rozprawy naukowe IHAR-PIB-2014 nr 48, str. 258-262.
  • Przetakiewicz J. 2012. Odporność jadalnych odmian ziemniaka na występujące w Polsce wirulentne patotypy grzyba Synchytrium endobioticum (Schilb.) Perc. Ziemniak Polski. 1: 26-28.
  • Przetakiewicz J. 2010. Harmonizacja oceny odporności odmian ziemniaka na porażenie przez grzyb Synchytrium endobioticum.Identyfikacja patotypów S. endobioticum.  Ziemniak Polski. Ziemniak Polski 4: 42-45.
  • Przetakiewicz J. 2010. Odporność polskich odmian ziemniaka na występujące w kraju wirulentne patotypy 2(Ch1) i 3(M1) grzyba Synchytrium endobioticum (Schilb.) Per. Biul. IHAR 257/258.
  • Przetakiewicz J. 2013. Effects of fungicide treatments of potato sprouts on resistance assessment to Synchytrium endobioticum (Schilb.) Perc. using the Glynne-Lemmerzahl method. Bulletin OEPP/EPPO Bulletin 43(2): 280-284.
  • Przetakiewicz J. 2014. Monitoring występowania nowych, agresywnych patotypów Synchytrium endobioticum z uwzględnieniem wykrycia ewentualnego pojawienia się nowych czynników wirulencji w populacjach patogena występujących w Polsce.Monografie i rozprawy naukowe IHAR-PIB-2014 nr 48, str. 263-271.
  • Przetakiewicz, J. 2008. Assessment of the resistance of potato cultivars to Synchytrium endobioticum (Schilb.) Per. in Poland. Bull. OEPP/EPPO Bull. 38: 211-215.
  • Przetakiewicz, J. 2014a. Manual of Security Sensitive Microbes and Toxins: Synchytrium endobioticum. ed. Dong Liu, 823-829. Boca Raton, London, New York: CRC Press.
  • Przetakiewicz, J. 2014b. First report of Synchytrium endobioticum (potato wart disease) pathotype 18(T1) in Poland. Plant Disease 98(5): 688.
  • Przetakiewicz, J. 2015. First report of new pathotype 39(P1) of Synchytrium endobioticum causing potato wart disease in Poland. Plant Disease 99(2): 285.2.
  • Zijlstra C., Lund I., Justesen A., Nicolaisen M., Jensen P.K., Bianciotto V., Posta K., Balestrini R., Przetakiewicz A., Czembor E., van de Zande J. 2011. Combining novel monitoring tools and precision application technologies. Pest Management Science 67: 616–625.
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