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Department of Genetic Engineering

Department of Genetic Engineering
Professor Waclaw Orczyk, D.Sc.
phone: (+48 22) 7334621;
e-mail: w.orczyk@ihar.edu.pl

Research projects:

  1. Histopathological analysis of plant-pathogen interaction and elucidation of oxidative burst role in expression of plant defense against pathogen.
  2. Identification of wheat transcripts regulated during infection of brown rust (Puccinia triticina).
  3. Elaboration of experimental tools for functional gene analysis - adaptation of BSMV-VIGS system for wheat, Triticale and rye functional genomics.
  4. Application of BSMV-VIGS experimental system for functional analysis of selected rye (Secale cereale) genes responsible for DIBOA biosynthesis.
  5. Application of transgenesis for analysis of gene function and for generation of wheat and barley plants with desired characteristics.
  6. Identification of brassinosteroid regulators in barley and analysis of their role in plant reaction for environmental stresses.

 

Selected publications:

·    Zalewski W, Orczyk W, Gasparis S, Nadolska-Orczyk A. 2012. HvCKX2gene silencing by biolistic or Agrobacterium-mediated transformation in barley leads to different phenotypes. BMC Plant Biology, 12:206; http://www.biomedcentral.com/1471-2229/12/206

·    Dmochowska-BogutaM., Nadolska-Orczyk A., Orczyk W. 2012. Roles of peroxidases and NADPH oxidases in the oxidative response of wheat (Triticum aestivum) to brown rust (Puccinia triticina) infection. Plant Pathol. Doi: 10.1111/ppa.12009

·    Binka A., Orczyk W., Nadolska-Orczyk A. 2012. The Agrobacterium-mediated transformation of common wheat (Triticum aestivum L.) and triticale (x Triticosecale Wittmack): role of the binary vector systems and selection cassettes. J. Appl. Genet. 2012. 53:1-8. DOI: 10.1007/s13353-011-0064-y.

·    Kawałek A, Dmochowska-Boguta M, Nadolska-Orczyk A, Orczyk W. 2012. Anew BSMV-based vector with modified β molecule allows simultaneous and stable silencing of two genes. Cell. Mol.Biol. Lett. 17: 107-123. DOI: 10.2478/s11658-011-0041-9, 17: 107-123.

·    Gasparis S., Orczyk W., Zalewski W., Nadolska-Orczyk A. 2011. The RNA-mediated silencing of one of the Pin genes in allohexaploid wheat simultaneously decreases the expression of the other, and increases grain hardness. J. Exp. Bot. 62 (11): 4025-4036. DOI:10.1093/jxb/err103

·    Zalewski W., Galuszka P., Gasparis S., Orczyk W., Nadolska-Orczyk A. 2010. Silencing of the HvCKX1 gene decreases the cytokinin oxidase/dehydrogenase level in barley and leads to higher plant productivity. J. Exp. Bot. 61: 1839-1851. DOI:10.1093/jxb/erq052

·    Nadolska-Orczyk A., Galuszka P., Zalewski W., Orczyk W. 2010. ADNA cassette, binary vector, and strain of A. tumefaciens and a method of producing cereal plant of increased productivity and/or root mass. International patent application No. PCT/PL2010/050019

·    Orczyk W., Dmochowska-Boguta M., Czembor H.J., Nadolska-Orczyk A. 2010. Spatiotemporal patterns of oxidative burst and micronecrosis in resistance of wheat to brown rust infection. Plant Pathol. 59: 567-575. DOI: 10.1111/j.1365-3059.2010.02257.x

·    Nadolska-Orczyk A., Gasparis S., Orczyk W. 2009. The determinants of grain texture in cereals. J. Appl. Genet. 50: 185-197.

·    Gasparis S., Bregier C., Orczyk W., Nadolska-Orczyk A. 2008. Agrobacterium-mediated transformation of oat (Avena sativa L) cultivars via immature embryo and leaf explants. Plant Cell Rep. 27: 1721-1729. DOI: 10.1007/s00299-008-0593-y

·    Nadolska-Orczyk A., Pietrusinska A., Binka-Wyrwa A., Kuc D, Orczyk W. 2007. Diploid potato (Solanum tuberosum L.) as a model crop to study transgene expression. Cell. Mol. Biol. Lett. 12: 206-219.

·    Przetakiewicz J., Nadolska-Orczyk A., Kuc D., Orczyk W. 2007. Tetraploid somatic hybrids of potato (Solanum tuberosumL.) obtained from diploid breeding lines. Cell. Mol. Biol. Lett. 12: 253-267.

·    Djukanovic V., Orczyk W., Gao H., Sun X., Garrett N., Zhen S., Gordon-Kamm W., Barton J., Lyznik L.A. 2006. Gene conversion in transgenic maize plants expressing FLP/FRT and Cre/loxP site-specific recombination systems. Plant Biotechnol. J. 4 (3), 345-357.

·    Bolibok H., Rakoczy-Trojanowska M., Wyrzykowska M., Radecka M., Orczyk W. 2006. Identyfication of microsatellite markers in the rye genome. Cell. Mol. Biol. Lett. 11: 291-298.

·    Nadolska-Orczyk A., Przetakiewicz A., Kopera K., Bińka A., Orczyk W. 2005. Efficient method of Agrobacterium-mediated transformation for triticale (x Triticosecale Wittmack). J. Plant Growth Reg. 24: 2-10.

·    Przetakiewicz A., Karaś A., Orczyk W., Nadolska-Orczyk A. 2004. Agrobacterium-mediated transformation of polyploid cereals. The efficiency of selection and transgene expression in wheat. Cell. Mol. Biol. Lett. 9: 903-917.

·    Nadolska-Orczyk A., Orczyk W. 2003. Agrobacterium-mediated transformation of cereals. In: Plant Genetic Engineering Vol 2: Improvement of Food Crops. (Ed. P.K. Jaiwal and R.P. Singh); Sci. Tech. Publishing LLC, USA, pp. 1-25.

·    Przetakiewicz A., Orczyk W., Nadolska-Orczyk A. 2003. The effect of auxin on plant regeneration of wheat, barley and triticale. Plant Cell, Tissue Org. Cult. 73: 245-256.

·    Orczyk W., Przetakiewicz J., Nadolska-Orczyk A. 2003. Somatic hybrids of Solanum tuberosum – application to genetics and breeding. Plant Cell, Tissue Org. Cult.73: 245-256.

·    Przetakiewicz J., Nadolska-Orczyk A., Orczyk W. 2002. The Use of RAPD and Semi-random Markers to Screen Somatic Hybrids Between Diploid Lines of Solanum tuberosum L. Cell and Molecular Biology Letters 7: 6711-676.

·    Nadolska-Orczyk A., Orczyk W. 2000. Study of the factors influencing Agrobacterium-mediated transformation of pea (Pisum sativum L.). Mol. Breed.6: 185-194.

·    Nadolska-Orczyk A., Orczyk W., Przetakiewicz A. 2000. Agrobacterium-mediated transformation of cereals – from technique development to its application. Acta Physiol. Plant.22: 77-88. 

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